Protein purification

Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the specification of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.

HNCA experiment

HNCA is a 3D triple-resonance NMR experiment commonly used in the field of protein NMR. The name derives from the experiment's magnetization transfer pathway: The magnetization of the amide proton of an amino acid residue is transferred to the amide

HNCOCA experiment

HNCOCA is a 3D triple-resonance NMR experiment commonly used in the field of protein NMR. The name derives from the experiment's magnetization transfer pathway: The magnetization of the amide proton of an amino acid residue is transferred to the amide

Proton-sensing G protein-coupled receptors

Proton-sensing G protein-coupled receptors are transmembrane receptors which sense acidic pH and include GPR132 (G2A), GPR4, GPR68 (OGR1) and GPR65 (TDAG8). These G protein-coupled receptors are activated when extracellular pH falls into the range of 6.4

Giulio Cantoni

Giulio Leonardo Cantoni was the director of the United States' National Institutes of Health's Laboratory of Cellular Pharmacology, later renamed the Laboratory of General and Comparative Biochemistry

Snubnose sculpin

The snubnose sculpin, Orthonopias triacis, is a fish of the sculpin family native to the eastern Pacific Ocean

Painted greenling

The painted greenling is a marine fish native to the northeast Pacific Ocean. Its range is from Kodiak Island, Alaska to central Baja California. It can reach a total size of 25 cm (10 in) and has seven vertical dark bands. It inhabits rocky areas

Stripedfin ronquil

The stripedfin ronquil is a fish native to the eastern Pacific Ocean. Its range is from San Francisco, California to northern Baja California, Mexico. It can reach a total length of 16 cm, and is elongated with a characteristic black and white pattern

Bathymaster signatus

Bathymaster signatus, the searcher, is a fish native to the Northern Pacific Ocean. Its range includes the East Siberian Sea to eastern Kamchatka and the Komandorski Islands, Russia to the state of Washington, United States including the Sea of Okhotsk

List of types of proteins

List of proteins contains large amounts of information concerning genes and proteins. Gene and genome sequencing projects have led to the creation of many gene databases and attempts to organize all of the available information. Classifications may be

Homotopic connectivity

In biology, homotopic connectivity is the connectivity between mirror areas of the human brain hemispheres